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quassan for sawfly

Posted by Michael Phillips 
quassan for sawfly
March 14, 2013 08:58AM
One of the project areas being looked at by the Research Committee is the use of quassan to deal with high rates of European Apple Sawfly infestation. Quassan (the active ingredient found in the wood of the quassia tree) is commonly used in organic orchards in Europe to deal with EAS at the first instar stage. A paper posted in the HON Library: European Apple Sawfly will bring you up to speed here. Growers throughout the northeast have this pest on their radar for sure.

We have a choice of importing the dry quassan powder from a German supplier, a liquid quassan formulation from a Swiss supplier, and/or simply ordering quassia wood from an herb supplier. The first two are "standardized" in that the percent active ingredient is known. That's good to know when striving to do legitmate research. On the other hand, the availability of the actual herb with which to make a straightforward decoction seems far more practical.

Quassia is indigenous to Brazil, Peru, Venezuela, Suriname, Colombia, Argentina, and Guyana. The herb is extremely bitter; it's one name amargo means "bitter" in Spanish. These small deciduous trees are noted for never being bothered by insects pests. This is due to a component in the resin called quassin, which has long been used by West Indies farmers as a botanical insecticide. Herbalists use a quassia extract primarily as a digestive aid and to treat liver and gallbladder problems. A beer brewer can use quassia as a substitute for hops.

Quassin is considered to be significantly more effective on the first instar stage of EAS because of “scratch ingestion” as the tiny larvae feed across the skin of a first fruitlet. The second instar stage penetrates a next fruitlet to consume the developing seeds. Unstopped, sawfly goes on from there to more fruitlets. You get the idea. The larger larvae hardly stop to ingest the treated surface ... thus application timing needs to be right tight to petal fall to gain an upper hand. Making such a "bitter spray" more systemic by means of fermented garlic extract might be the ticket to improve efficacy on the second instar.

Lost Nation Orchard
Zone 4b in New Hampshire



Edited 2 time(s). Last edit at 04/03/2013 07:06PM by Michael Phillips.
Help! I need a statistician.
August 04, 2014 03:22PM
I now have 4 years' worth of data on the effectiveness of Quassia in controlling EAS. Eyeballing my results it is apparent that Quassia extract is effective. But I need to establish that this is not due to chance. I think that what
I need is a t-test of the difference of the means, (ie null hypothesis that the mean damage of the control and treated trees are the same.) But I am hung up on how to deal with small sample numbers of some observations, ie. the standard deviations of different observations will vary widely, and hence may not be amenable to legitimate combination. I don't really expect that any of our merry band of amateur researchers will hold the skills needed to advise me, but just in case, does anybody out there feel able to advise me on statistical methods?

Broomholm Orchard
Zone 5b in Nova Scotia
Re: quassan for sawfly
March 13, 2015 07:28PM
I have reached a bit of an impasse with this, and would welcome any and all comments, suggestions or expressions of interest. Here is where I stand: I started 4 years ago, spraying one of each paired cultivar in my very small orchard (of multiple cultivars) with a commercial Quassia extract (from Germany), brought in after massive paperwork, as a formal research project. That first year I was working with a scientist from a government research station. For reasons I have never been able to determine, she dumped me unceromoniously after that first year, and will no longer even return attempted communications. Since then I have carried on, using a dwindling supply of my German extract to repeat the experiment - spraying half the trees with Quassia, and leaving the other matching cultivar unsprayed as a control. Now, if you have followed me to this point, you will appreciate that my sample sizes are awfully small - generally one pair of trees of each cultivar. My fellow researcher was using a block of a single cultivar, with multiple instances of sprayed and control trees. In simple terms, we both demonstrated that Quassia amara extract is very effective in controlling European Apple Sawfly, reducing damage from as much as 50% down to 3% or less. But my own trials, while flawed by very small samples, have a positive aspect: it is apparent that different cultivars vary markedly in susceptibility to EAS attack, (ranging from almost complete resistance to up to 50%)

Now, I suspect that one of the causes of my partner losing interest had to do with the issue of registration of pesticides. She took a request to the government agency which approves so-called "minor use" products - products which are permitted to be used despite their not having being subject to a full formal application for registration. It was turned down. So there is no legal means to import and/or use Quassia extract, at least in Canada. Nor is it formally registered in Europe. The difference between Europe and Canada is that in Europe the organic certification folk permit growers to use products other than those which are formally registered, without losing their organic certification, and the government food protection folk agree not to interfere as long as the organic certifiers are satisfied. In Canada, it is the other way around - the Pest Management Regulatory Agency has primacy, and the organic folk prohibit anything which is not approved by them first.

It is apparent that I am not going to get anywhere in trying to gain approval in Canada for the commercial extracts. And I suspect that the same situation will apply in the States. BUT, there is another avenue - bring in the raw wood chips, and brew one's own "Quassia tea" on farm. I put this to the organic certification folk, and they responded that they did not know how to answer. I pointed out to them that they had no problem with compost tea and/or nettle tea, and that a tea made from Quassia wood chips is arguably no different. Their first reaction was that Quassia is not on the list of approved materials. (Compost tea actually is officially listed; nettle tea is not) I then found the following "permitted" use:
"Substances that protect plants from harsh environmental conditions such as frost and sunburn, infection, the buildup of dirt on leaf surfaces, or injury by a pest. Natural substances are allowed, including but not limited to calcium carbonate, diatomaceous earth, kaolin clay, pine oil, pine resin and yucca." (Bold my own emphasis) (Parenthetically, neem oil is not registered in Canada, and for the most part the organic certification folk have refused to permit its use under their standards, "because it isn't on the list".)
This is when they got spooked and stopped responding to me, yea or nay. But they have said they will think about it, and get back to me. (That was 2 years ago.)

I figured I had beaten down their resistance, at least enough that they could not pull rank and block my access on the basis of "importation of an unregistered pesticide". But then I ran up against Border Services regulations, which, in essence, forbid the importation of any and all woods, bark, or other plant products unless explicitly listed in a list of permitted materials, (which, of course, does not include Quassia amara specifically).

With this impossibly long preamble, let me lay out what I want to do next:

1) I do think that my sample size is too small to make terribly valid conclusions. So if I can enlist a few intrepid growers with more trees willing to replicate my experiments, it would be well worthwhile. (Note that this involves leaving some trees unsprayed, with the sacrifice of some portion of the crop to the varmints (EAS). But if you are currently sacrificing all your trees to the EAS, saving half of them from damage is still a lot better.)

2) I do not think trying to get prepared extract brought in is a viable course. But farm-prepared extracts may well be a feasible plan. My sense is that the Canadian bureaucracy may be insurmountable, but, as Michael notes, there is a firm in Miami which is currently importing bark chips into the States already. If anybody is inspired to collaborate with me, I would suggest that we organise a purchase of bark chips from this vendor, (who will have already worked through all bureaucratic import hurdles), and do a full-scale trial with home-made extract.

3) My sense is that I have run up against a confounding variable - different cultivars have markedly different susceptibilities to attack by EAS, (unexplained by location in the orchard, time of bloom, or any other variable I could think of). I think, from the viewpoint of Quassia vs. EAS, the only option is to stick to what amounts to 20 or 30 individual experiments, treating each pair of trees as individual trials, (or, in a few instances, small groups of 2 or 3 trees, where I have more than 2 trees of a given cultivar.) But this also raises a completely different research question: Is this inter-cultivar susceptibility a general phenomenon? This harkens back to Michael's current project (if I have correctly interpreted it - it is a littele unclear from his brief message just what he is up to) - susceptibility of different cultivars to CAR. And there is no particular reason why I couldn't piggyback on Michael's collaborations if others are willing - monitor and record which cultivars are susceptible and which resistant to CAR. And at the same time document the extent of attack by EAS.

Michael quite appropriately is looking for others to drive their own individual projects. And I am very much willing to take charge of both of the above projects - effectivelness of Quassia against EAS, and natural resistance of different cultivars to EAS. All I need is willing collaborators. So if any of you are moved to work with me on pursuing either (or both!) of these efforts, I would strongly urge you to send me a message, and we will get on it right away.

Broomholm Orchard
Zone 5b in Nova Scotia
Re: quassan for sawfly
May 05, 2015 06:51PM
David, we covered some ground a few years ago on this and I dropped the ball. Apologies! I am very interested on many levels with the use of Quassia for EAS control. It is too late this year for my collaboration (all of my growers' trees are heading into full bloom) but I would love to establish some state-side research projects for 2016 looking at exacttly what you've asked: the varietal susceptibility.

One thing I could find out if I had time to read, but I don't, so I'll ask is: does anybody 1) understand the attraction (e.g., specific volatile compounds) of certain culivars or 2) the active compound in Quassia that is so effective? Couldnt we grow it here? Is there a surrogate plant with a similar compund?

Mike

Mike Biltonen, Know Your Roots
Zone 5b in New York
Re: quassan for sawfly
May 06, 2015 08:36AM
In answer to question 1, yes, there is research on the specific volatile compounds which both attract specific pests and actually repel others, and I have a couple of papers in the jumble on my desk, which I couldn't immediately lay my hands on, but would be happy to track down if you really want to know. (Having found this information awhile ago, I wasn't entirely sure what it meant in practical terms.)
Question #2 This one is much clearer, as Jutte Kienzle explicitly studied it. The ingredient which is most toxic to the EAS is quassin, with a lesser effect of neoquassin. (I can give you the chemical structures of both of these if you want, but they are basically simple cyclic molecules. Methods of synthesizing them have been published, (but nobody has expressed an interest in doing so commercially). Indeed it was Kienzle's research which led to the standardisation of the concentration of quassin in the commercial preparations, as some growers brewing their own quassia extract were not getting good control. Kienzle showed that the concentraion of quassin in these extracts was low. She went on to test varying concentrations of quassin, and determined that a minimum of 5 (I forget the units) per square metre of tree canopy was necessary. (I can post the proper, accurate details if anybody is really interested. It just requires my going back into my files. But I am dashing this off in some haste, with just the essence of the thing.)

Broomholm Orchard
Zone 5b in Nova Scotia
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